Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Optimization of the background electrolyte (BGE) composition of the CE–MS method for triptorelin determination.

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques:

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Representative parent ion ( a ) and product ion ( b ) triple quadrupole mass spectra of triptorelin. The spectral profiles indicate qualifier ( m/z 249.0) and quantifier ( m/z 328.3) product ions serving for unequivocal identification and quantification of triptorelin. The fragmentor voltage was set at 160 V and the collision energy was 20 eV.

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques:

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Comparison of selected validation parameters of the CE–MS methods for triptorelin in model water matrix.

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques:

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Effect of the CE injection mode (hydrodynamic vs. electrokinetic) on the analytical signal intensity. Concentration of triptorelin in the analyzed model sample was 0.5 µg mL −1 . ( a ) Hydrodynamic injection was performed at 50 mbar for 20 s. ( b ) The electrokinetic injection (FESI) was performed at 10 kV for 20 s after the hydrodynamic introduction of a short water plug (50 mbar for 100 s) into the capillary. For other details of the CE–MS method see .

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques: Injection, Concentration Assay

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Operation and calibration parameters of the MSI/FESI–CE–MS/MS method for determination of triptorelin in model water and plasma samples.

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques:

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Accuracy and precision of the CE–MS/MS method for triptorelin in water/saline and plasma QC samples.

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques:

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Representative multiple reaction monitoring (MRM) records obtained from the MSI/FESI–CE–MS analysis of ( a ) blank water (saline) sample, ( b ) triptorelin at the LOQ concentration level (10 ng mL −1 ) in water, ( c ) blank plasma sample, and ( d ) triptorelin at the LOQ concentration level (50 ng mL −1 ) in plasma. The records were obtained for the m/z transition 656.5→328.3. For other details of the CE–MS method see .

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques: Concentration Assay

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: Stability testing of triptorelin in plasma QC samples.

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques:

Journal: Biomedicines

Article Title: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

doi: 10.3390/biomedicines9101488

Figure Lengend Snippet: MSI-CE-MS analysis of ( a ) 100-times diluted pharmaceutical sample Diphereline ® 0.1 mg (powder for injection), and ( b ) plasma sample spiked with triptorelin standard at 0.1 µg mL −1 concentration level. The records were obtained for the m/z transition 656.5→328.3. For more details of the CE–MS method see .

Article Snippet: Triptorelin acetate was purchased from Caslo (Lyngby, Denmark) and the commercial drug Diphereline ® 0.1 mg (containing triptorelin acetate powder for injection) was obtained from Ipsen Pharma Biotech (Signes, France).

Techniques: Injection, Concentration Assay